show Abstracthide AbstractWe performed RNA-seq for WT and foxo mutant flies, by combining with Dr. Baihua's foxo chip-seq data, we found 101 foxo-repressed and 300 foxo-activated genes. By analyzing expression change of these genes during age, we found foxo-repressed genes became activated, while foxo-activated genes became repressed, suggesting foxo signaling declines with age. Overall design: Find potential foxo-regulated genes by comparing transcriptome differences between WT and foxo mutant flies, analyze their changes with age using RNA-sequencing data performed at 3d, 15d, 30d and 45d age of WT flies.